Human galectin-9 ELISA kit (Gal-9) is a Sandwich (quantitative) ELISA kit for the measurement of Human galectin-9 (Gal-9) in Human in Cell culture supernatant, Plasma, Serum samples.
Be the first to review this product! Submit a review
Images

Publications
Filter by
1 Publication for All applications
In vivo (Athens, Greece) 37:2577-25842023
Evaluation of the Relationship Between miRNA-22-3p and Gal-9 Levels in Glioblastoma.
Applications
:
Unspecified application
Reactive species
:
Unspecified reactive species
Zerrin Barut,Fatma Tuba Akdeniz
PubMed 37905655
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
15.6 - 500 pg/mL
- Assay time
3h 45m
- Sensitivity
= 8 pg/mL
Reactivity data
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
See full target information LGALS9
Function
Binds galactosides (PubMed:18005988). Has high affinity for the Forssman pentasaccharide (PubMed:18005988). Ligand for HAVCR2/TIM3 (PubMed:16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed:16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed:23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed:20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed:16116184, PubMed:24465902). Inhibits degranulation and induces apoptosis of mast cells (PubMed:24465902). Induces maturation and migration of dendritic cells (PubMed:16116184, PubMed:25754930). Inhibits natural killer (NK) cell function (PubMed:23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed:25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity).Isoform 2Acts as an eosinophil chemoattractant (PubMed:9642261). It also inhibits angiogenesis (PubMed:24333696). Suppresses IFNG production by natural killer cells (By similarity).
Alternative names
Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9
What's included?
1 x 96 Tests
Components
Biotinylated Antibody Gal-9
1 x 400 µL
Biotinylated Antibody diluent
1 x 7 mL
Gal-9 Coated Microwell strips
1 x 1 Unit
Gal-9 Standard
2 x 1 Vial
HRP Diluent
1 x 12 mL
Plastic Plate Cover
2 x 1 Unit
Standard diluent Buffer
1 x 15 mL
Stop Reagent
1 x 11 mL
Streptavidin-HRP
2 x 5 µL
TMB Substrate
1 x 11 mL
Wash Buffer
1 x 10 mL
Recommended products
Human galectin-9 ELISA kit (Gal-9) is a Sandwich (quantitative) ELISA kit for the measurement of Human galectin-9 (Gal-9) in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
15.6 - 500 pg/mL
- Assay time
3h 45m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 8 pg/mL
Precision
Intra assay
Sample Various n 18 mean - SD - C.V. = 3.2Sample n mean SD C.V.
Inter assay
Sample Sample 1 n mean 345.43 pg/mL SD 15.45 C.V. 4.5 Sample Sample 2 n mean 189.62 pg/mL SD 25.4 C.V. 13.4 Sample Sample 3 n mean 461.78 pg/mL SD 11.51 C.V. 2.5 Sample Sample 4 n mean 249.55 pg/mL SD 13.28 C.V. 5.3 Sample Sample 5 n mean 589.98 pg/mL SD 8.15 C.V. 1.4 Sample Sample 6 n mean 296.4 pg/mL SD 21.38 C.V. 7.2Sample n mean SD C.V.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Galectin-9 ELISA kit (Gal-9) (ab273161) is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of Galectin-9 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human Galectin-9.
A capture antibody highly specific for Galectin-9 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Galectin-9 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-Galectin-9 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Galectin-9 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Galectin-9 in any sample tested.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-9 also known as Gal-9 or LGALS9 is a β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.
- Biological function summary
Galectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.
- Pathways
Galectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.
- Associated diseases and disorders
Galectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
.
1 product image
ELISA - Human galectin-9 ELISA kit (Gal-9) (ab273161)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Human Galectin-9 ELISA kit (Gal-9) standard curve.
Downloads
Product protocols
For this product, it's our understanding that no specific protocols are required. You can:
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contactpartnerships@abcam.com